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antibodies against cd13  (Proteintech)


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    Proteintech antibodies against cd13
    Antibodies Against Cd13, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+cd13/CD13+Antibody/10__1097_slash_js9__0000000000004068-122-24-28
    Average 93 stars, based on 32 article reviews
    antibodies against cd13 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: ANPEP as a potential mediator linking aspartame exposure to MAFLD: insights from network toxicology and multi-omics analyses
    Article Snippet: .. For multicolor immunofluorescence (mIF), sections were processed with antigen retrieval and blocking, followed by automated staining (AlphaXPainter® X30, Alpha X Bio, Beijing, China) using antibodies against CD13 (14553-1-AP, Proteintech), CD68 (25747-1-AP, Proteintech), and additional panel markers. .. Signals were visualized with the Alpha X 7-Color IF Kit, nuclei counterstained with DAPI, scanned using ZEISS AXIOSCAN 7 (ZEISS, Germany), and analyzed with Image-Pro Plus 6.0.

    Blocking Assay:

    Article Title: ANPEP as a potential mediator linking aspartame exposure to MAFLD: insights from network toxicology and multi-omics analyses
    Article Snippet: .. For multicolor immunofluorescence (mIF), sections were processed with antigen retrieval and blocking, followed by automated staining (AlphaXPainter® X30, Alpha X Bio, Beijing, China) using antibodies against CD13 (14553-1-AP, Proteintech), CD68 (25747-1-AP, Proteintech), and additional panel markers. .. Signals were visualized with the Alpha X 7-Color IF Kit, nuclei counterstained with DAPI, scanned using ZEISS AXIOSCAN 7 (ZEISS, Germany), and analyzed with Image-Pro Plus 6.0.

    Staining:

    Article Title: ANPEP as a potential mediator linking aspartame exposure to MAFLD: insights from network toxicology and multi-omics analyses
    Article Snippet: .. For multicolor immunofluorescence (mIF), sections were processed with antigen retrieval and blocking, followed by automated staining (AlphaXPainter® X30, Alpha X Bio, Beijing, China) using antibodies against CD13 (14553-1-AP, Proteintech), CD68 (25747-1-AP, Proteintech), and additional panel markers. .. Signals were visualized with the Alpha X 7-Color IF Kit, nuclei counterstained with DAPI, scanned using ZEISS AXIOSCAN 7 (ZEISS, Germany), and analyzed with Image-Pro Plus 6.0.

    Incubation:

    Article Title: Suppressing mortality and curbing cellular damage by targeting VHL
    Article Snippet: .. The sections were incubated with primary antibodies against CD13 (Proteintech, 66211-1-Ig), NeuN (ABclonal, A19086), SMI312 (BioLegend, 837904) for 12 h at 4 °C and subsequently treated with fluorescence-conjugated secondary antibody (Beyotime, A0423 and A0460) for 2 hr at room temperature in the dark. .. To visualize brain microvessels, sections were incubated with Lycopersicon Esculentum DyLight 488 (Invitrogen, L32470) for 1 hr.

    Article Title: Suppressing APOE4-induced neural pathologies by targeting the VHL–HIF axis
    Article Snippet: .. The sections were incubated with primary antibodies against CD13 (Proteintech, 66211-1-Ig), NeuN (ABclonal, A19086), SMI312 (BioLegend, 837904) for 12 h at 4 °C and subsequently treated with fluorescence-conjugated secondary antibody (Beyotime, A0423 and A0460) for 2 hr at room temperature in the dark. .. To visualize brain microvessels, sections were incubated with Lycopersicon Esculentum DyLight 488 (Invitrogen, L32470) for 1 hr.

    Fluorescence:

    Article Title: Suppressing mortality and curbing cellular damage by targeting VHL
    Article Snippet: .. The sections were incubated with primary antibodies against CD13 (Proteintech, 66211-1-Ig), NeuN (ABclonal, A19086), SMI312 (BioLegend, 837904) for 12 h at 4 °C and subsequently treated with fluorescence-conjugated secondary antibody (Beyotime, A0423 and A0460) for 2 hr at room temperature in the dark. .. To visualize brain microvessels, sections were incubated with Lycopersicon Esculentum DyLight 488 (Invitrogen, L32470) for 1 hr.

    Article Title: Suppressing APOE4-induced neural pathologies by targeting the VHL–HIF axis
    Article Snippet: .. The sections were incubated with primary antibodies against CD13 (Proteintech, 66211-1-Ig), NeuN (ABclonal, A19086), SMI312 (BioLegend, 837904) for 12 h at 4 °C and subsequently treated with fluorescence-conjugated secondary antibody (Beyotime, A0423 and A0460) for 2 hr at room temperature in the dark. .. To visualize brain microvessels, sections were incubated with Lycopersicon Esculentum DyLight 488 (Invitrogen, L32470) for 1 hr.



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    Brain pericytes increase glutamate uptake in astrocyte cultures. (A) Double immunofluorescence staining for <t>CD13/EAAT1</t> (top left panel), CD13/EAAT2 (top right panel), GFAP/EAAT1 (bottom left panel), and GFAP/EAAT2 (bottom right panel) in intact mouse hippocampus sections. CD13 and GFAP signals appear green, while EAAT1 and EAAT2 signals appear red. (B) Regression analyses of cell/medium ratios of [ 3 H]-L-glutamate ([ 3 H]-L-Glu) uptake versus incubation time in astrocytes cultured without pericytes (astrocyte monocultures) or with pericytes (pericyte co-cultures). Astrocytes in monocultures and pericyte co-cultures were incubated in uptake buffer containing [ 3 H]-L-Glu for 1, 2, 5, and 10 min. A linear regression analysis was also performed to obtain the rate of [ 3 H]-L-Glu uptake. The slope of the lines was 9.48 in astrocyte monocultures ( r 2 = 0.90) and 13.54 in pericyte co-cultures ( r 2 = 0.89). Values are expressed as means ± SEM (n = 4 per point). * p < 0.05, between astrocyte monocultures and pericyte co-cultures.
    Antibodies Against Cd13, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    antibodies against cd13  (Proteintech)
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    Brain pericytes increase glutamate uptake in astrocyte cultures. (A) Double immunofluorescence staining for <t>CD13/EAAT1</t> (top left panel), CD13/EAAT2 (top right panel), GFAP/EAAT1 (bottom left panel), and GFAP/EAAT2 (bottom right panel) in intact mouse hippocampus sections. CD13 and GFAP signals appear green, while EAAT1 and EAAT2 signals appear red. (B) Regression analyses of cell/medium ratios of [ 3 H]-L-glutamate ([ 3 H]-L-Glu) uptake versus incubation time in astrocytes cultured without pericytes (astrocyte monocultures) or with pericytes (pericyte co-cultures). Astrocytes in monocultures and pericyte co-cultures were incubated in uptake buffer containing [ 3 H]-L-Glu for 1, 2, 5, and 10 min. A linear regression analysis was also performed to obtain the rate of [ 3 H]-L-Glu uptake. The slope of the lines was 9.48 in astrocyte monocultures ( r 2 = 0.90) and 13.54 in pericyte co-cultures ( r 2 = 0.89). Values are expressed as means ± SEM (n = 4 per point). * p < 0.05, between astrocyte monocultures and pericyte co-cultures.
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    antibody against cd13  (Bio-Rad)
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    Brain pericytes increase glutamate uptake in astrocyte cultures. (A) Double immunofluorescence staining for <t>CD13/EAAT1</t> (top left panel), CD13/EAAT2 (top right panel), GFAP/EAAT1 (bottom left panel), and GFAP/EAAT2 (bottom right panel) in intact mouse hippocampus sections. CD13 and GFAP signals appear green, while EAAT1 and EAAT2 signals appear red. (B) Regression analyses of cell/medium ratios of [ 3 H]-L-glutamate ([ 3 H]-L-Glu) uptake versus incubation time in astrocytes cultured without pericytes (astrocyte monocultures) or with pericytes (pericyte co-cultures). Astrocytes in monocultures and pericyte co-cultures were incubated in uptake buffer containing [ 3 H]-L-Glu for 1, 2, 5, and 10 min. A linear regression analysis was also performed to obtain the rate of [ 3 H]-L-Glu uptake. The slope of the lines was 9.48 in astrocyte monocultures ( r 2 = 0.90) and 13.54 in pericyte co-cultures ( r 2 = 0.89). Values are expressed as means ± SEM (n = 4 per point). * p < 0.05, between astrocyte monocultures and pericyte co-cultures.
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    antibodies against cd90, cd73, cd13, cd45, hla-dr  (Becton Dickinson)
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    Becton Dickinson antibodies against cd90, cd73, cd13, cd45, hla-dr
    Human amnion-derived mesenchymal stromal/stem cells (hAMSCs) were grown as monolayers with or without priming. (A) Representative DIC images of hAMSCs grown as monolayers without priming (hAMSCs), cultured under hypoxic conditions (hAMSCs after hypoxia), or treated with IFN-γ (hAMSCs after IFN-γ treatment). (B) Representative images of flow cytometry analysis for quantification of hAMSCs at step 2 for both positive (CD90, <t>CD73</t> and CD13) and negative surface markers (HLA-DR and CD45). Green represents the isotype control, and red represents stained cells. (C) Experimental plan and exosome characterization (size and concentration). DIC, differential interference contrast.
    Antibodies Against Cd90, Cd73, Cd13, Cd45, Hla Dr, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Brain pericytes increase glutamate uptake in astrocyte cultures. (A) Double immunofluorescence staining for CD13/EAAT1 (top left panel), CD13/EAAT2 (top right panel), GFAP/EAAT1 (bottom left panel), and GFAP/EAAT2 (bottom right panel) in intact mouse hippocampus sections. CD13 and GFAP signals appear green, while EAAT1 and EAAT2 signals appear red. (B) Regression analyses of cell/medium ratios of [ 3 H]-L-glutamate ([ 3 H]-L-Glu) uptake versus incubation time in astrocytes cultured without pericytes (astrocyte monocultures) or with pericytes (pericyte co-cultures). Astrocytes in monocultures and pericyte co-cultures were incubated in uptake buffer containing [ 3 H]-L-Glu for 1, 2, 5, and 10 min. A linear regression analysis was also performed to obtain the rate of [ 3 H]-L-Glu uptake. The slope of the lines was 9.48 in astrocyte monocultures ( r 2 = 0.90) and 13.54 in pericyte co-cultures ( r 2 = 0.89). Values are expressed as means ± SEM (n = 4 per point). * p < 0.05, between astrocyte monocultures and pericyte co-cultures.

    Journal: IBRO Neuroscience Reports

    Article Title: Brain pericytes upregulate glutamate uptake by astrocytes in vitro through sodium-dependent transporter

    doi: 10.1016/j.ibneur.2025.05.017

    Figure Lengend Snippet: Brain pericytes increase glutamate uptake in astrocyte cultures. (A) Double immunofluorescence staining for CD13/EAAT1 (top left panel), CD13/EAAT2 (top right panel), GFAP/EAAT1 (bottom left panel), and GFAP/EAAT2 (bottom right panel) in intact mouse hippocampus sections. CD13 and GFAP signals appear green, while EAAT1 and EAAT2 signals appear red. (B) Regression analyses of cell/medium ratios of [ 3 H]-L-glutamate ([ 3 H]-L-Glu) uptake versus incubation time in astrocytes cultured without pericytes (astrocyte monocultures) or with pericytes (pericyte co-cultures). Astrocytes in monocultures and pericyte co-cultures were incubated in uptake buffer containing [ 3 H]-L-Glu for 1, 2, 5, and 10 min. A linear regression analysis was also performed to obtain the rate of [ 3 H]-L-Glu uptake. The slope of the lines was 9.48 in astrocyte monocultures ( r 2 = 0.90) and 13.54 in pericyte co-cultures ( r 2 = 0.89). Values are expressed as means ± SEM (n = 4 per point). * p < 0.05, between astrocyte monocultures and pericyte co-cultures.

    Article Snippet: Antibodies against CD13 were obtained from Acris Antibodies (#SM2298P; Rockville, MD, USA).

    Techniques: Double Immunofluorescence Staining, Incubation, Cell Culture

    Human amnion-derived mesenchymal stromal/stem cells (hAMSCs) were grown as monolayers with or without priming. (A) Representative DIC images of hAMSCs grown as monolayers without priming (hAMSCs), cultured under hypoxic conditions (hAMSCs after hypoxia), or treated with IFN-γ (hAMSCs after IFN-γ treatment). (B) Representative images of flow cytometry analysis for quantification of hAMSCs at step 2 for both positive (CD90, CD73 and CD13) and negative surface markers (HLA-DR and CD45). Green represents the isotype control, and red represents stained cells. (C) Experimental plan and exosome characterization (size and concentration). DIC, differential interference contrast.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Proteomic analysis and functional validation reveal distinct therapeutic capabilities related to priming of mesenchymal stromal/stem cells with IFN-γ and hypoxia: potential implications for their clinical use

    doi: 10.3389/fcell.2024.1385712

    Figure Lengend Snippet: Human amnion-derived mesenchymal stromal/stem cells (hAMSCs) were grown as monolayers with or without priming. (A) Representative DIC images of hAMSCs grown as monolayers without priming (hAMSCs), cultured under hypoxic conditions (hAMSCs after hypoxia), or treated with IFN-γ (hAMSCs after IFN-γ treatment). (B) Representative images of flow cytometry analysis for quantification of hAMSCs at step 2 for both positive (CD90, CD73 and CD13) and negative surface markers (HLA-DR and CD45). Green represents the isotype control, and red represents stained cells. (C) Experimental plan and exosome characterization (size and concentration). DIC, differential interference contrast.

    Article Snippet: For phenotypic characterization, hAMSCs were first washed twice with FACS buffer (PBS containing 0.3% BSA and 0.1% NaN 3 ) and then incubated on ice for 30 min with specific antibodies against CD90, CD73, CD13, CD45, and HLA-DR (BD Biosciences, United States).

    Techniques: Derivative Assay, Cell Culture, Flow Cytometry, Staining, Concentration Assay